Glycerol on Lipid Enhancement and FAME Characterization in Algae for Raw Material of Biodiesel

Anwesha Khanra, Shrasti Vasistha, Monika Prakash Rai


To reduce the cost of media components and consecutively enhance the amount of lipid formation, the effect of crude glycerol on Euglena gracilis is reported for the first time. Presently, E. gracilis has been chosen because of excellent capability of its growth and lipid synthesis in mixotrophic culture condition on organic carbon sources (Glucose and Glycerol). Biochemical composition of the cell in presence of both organic carbon sources were compared in mixotrophic condition. Glycerol was selected as most suitable carbon source and the highest biomass concentration of 2.63 g L-1 and lipid accumulation of 27.64 % were observed. The effect of biodiesel derived crude glycerol on the growth of E. gracilis was found very positive. A significant increase in lipid accumulation (49.46%) was noticed in presence of crude glycerol by optimizing concentration and other culture parameters. Fatty acid methyl esters (FAMEs) produced from lipid biomolecules by following transesterification reaction, were analysed through GC-MS. FAMEs governed by 93.458% C16-C18 fatty acids with appropriate quantities of Saturated fatty acids (SFA) and Unsaturated fatty acids (UFA). Several requisite fuel properties were estimated and found to be in accordance with American and European biodiesel standards. Hence, this study focuses the improved lipid synthesis in E. gracilis utilizing crude glycerol for the preparation of raw biodiesel and it supports biorefinery approach.

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Algae, biomass energy, Mixotrophy

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